{"id":7643,"date":"2019-11-19T13:47:00","date_gmt":"2019-11-19T04:47:00","guid":{"rendered":"https:\/\/www.arkray-adams-portal.com\/?p=7643"},"modified":"2025-11-04T10:47:21","modified_gmt":"2025-11-04T01:47:21","slug":"sample-transport-and-or-storage-can-cause-falsely-low-hba1c-levels-in-blood-cells-measured-by-enzymatic-assay","status":"publish","type":"post","link":"https:\/\/www.arkray-adams-portal.com\/en_id\/sample-transport-and-or-storage-can-cause-falsely-low-hba1c-levels-in-blood-cells-measured-by-enzymatic-assay\/","title":{"rendered":"Sample transport and\/or storage can cause falsely low HbA1c levels in blood cells measured by enzymatic assay"},"content":{"rendered":"
\n
\n
<\/span>
\n
<\/div>\n\n\n\n
<\/div>\n\n\n\n
<\/div>\n\n\n\n

Home<\/span><\/a> > Articles<\/span><\/a> > Sample transport and\/or storage can cause falsely low HbA1c levels in blood cells measured by enzymatic assay<\/span><\/h2><\/div>\n<\/div><\/div>\n<\/div>\n\n\n\n
\n

Sample transport and\/or storage can cause falsely low HbA1c levels in blood cells measured by enzymatic assay<\/span><\/h2>\n\n\n\n
\n
\n
\n

https:\/\/doi.org\/10.1007\/s13340-019-00416-7<\/a><\/p>\n\n\n\n

Received: June 27, 2019; accepted December 31, 2019;<\/p>\n\n\n\n

published online November 19, 2019<\/p>\n<\/div>\n\n\n\n

\n
Download PDF<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n
\n

Abstract<\/h2><\/div>\n\n\n\n
\n
\n
\n

Although HbA1c measurement by enzymatic assay (EA-HbA1c) is widely used in health-screening settings in Japan, recent studies have suggested lower EA-HbA1c levels as compared with HbA1c levels measured by high-performance liquid chromatography (HPLC-HbA1c). Hypothesizing that falsely low levels of EA-HbA1c are attributable to hemolysis caused by sample transport and\/or storage, we measured EA-HbA1c in blood cells and whole blood after sample transport and compared them with HPLC-HbA1c levels. Blood samples were collected from ten non-diabetic individuals into sodium fluoride-containing blood collection tubes and immediately measured for EA-HbA1c in blood cells. After transport, the blood samples were again subjected to measurement of EA-HbA1c levels in blood cells and whole blood the following day. These EA-HbA1c levels were compared with HPLC-HbA1c levels. EA-HbA1c levels in blood cells measured immediately after sample collection did not significantly differ from HPLC-HbA1c levels. Transported blood samples showed hemolysis and significantly lower EA-HbA1c levels in blood cells, as compared with HPLC-HbA1c levels, whereas no significant difference was observed between EA-HbA1c levels in whole blood and HPLC-HbA1c levels. Transported blood samples showed hemolysis and falsely low EA-HbA1c levels in blood cells. Hemolysis caused by sample transport and\/or storage might be responsible for the falsely low EA-HbA1c levels. This should be kept in mind, because falsely low HbA1c levels may lead to a false-negative diagnosis of diabetes.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

\n
\n
\n

Related Links<\/h4><\/div><\/div><\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n
\n
\n
\n
\n
\n
\n
<\/path><\/svg><\/span>
HA-8190V<\/div><\/a><\/div><\/div>\n<\/div><\/div>\n<\/div>\n\n\n\n
\n
<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n
\n
\n
\n
\n
<\/div><\/div>\n<\/div>\n\n\n\n
\n
<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

https:\/\/doi.org\/10.1007\/s13340-019-00416-7 Received: June 27, 2019; accepted December 31, 2019; published online November 19, 2019 Abstract […]<\/p>\n","protected":false},"author":9,"featured_media":7760,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_eb_attr":"","_oasis_is_in_workflow":0,"_oasis_original":0,"_uag_custom_page_level_css":".banner{\n\tmargin: 0;\n\tpadding: 0;\n}\n\n.header-heading span:hover{\n\ttext-decoration: none!important;\n}\n\n.link{\n\ttext-decoration: underline;\n\ttext-decoration-color: blue;\n}\n\n.link:hover{\n\ttext-decoration: none!important;\n}\n\n.link span:hover{\n\ttext-decoration: none!important;\n}\n\n.separator{\n\twhite-space: nowrap;\n}\n\n.downloadPDF a:hover {\n\tbackground-color: #888888!important;\n}\n","footnotes":""},"categories":[49],"tags":[],"class_list":["post-7643","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publication"],"acf":[],"uagb_featured_image_src":{"full":["https:\/\/www.arkray-adams-portal.com\/wp-content\/uploads\/2025\/01\/pixabay_book.jpg",1280,853,false],"thumbnail":["https:\/\/www.arkray-adams-portal.com\/wp-content\/uploads\/2025\/01\/pixabay_book-150x150.jpg",150,150,true],"medium":["https:\/\/www.arkray-adams-portal.com\/wp-content\/uploads\/2025\/01\/pixabay_book-300x200.jpg",300,200,true],"medium_large":["https:\/\/www.arkray-adams-portal.com\/wp-content\/uploads\/2025\/01\/pixabay_book-768x512.jpg",768,512,true],"large":["https:\/\/www.arkray-adams-portal.com\/wp-content\/uploads\/2025\/01\/pixabay_book-1024x682.jpg",1024,682,true],"1536x1536":["https:\/\/www.arkray-adams-portal.com\/wp-content\/uploads\/2025\/01\/pixabay_book.jpg",1280,853,false],"2048x2048":["https:\/\/www.arkray-adams-portal.com\/wp-content\/uploads\/2025\/01\/pixabay_book.jpg",1280,853,false],"trp-custom-language-flag":["https:\/\/www.arkray-adams-portal.com\/wp-content\/uploads\/2025\/01\/pixabay_book-18x12.jpg",18,12,true]},"uagb_author_info":{"display_name":"Kazuko_c","author_link":"https:\/\/www.arkray-adams-portal.com\/en_id\/author\/kazuko_c\/"},"uagb_comment_info":0,"uagb_excerpt":"https:\/\/doi.org\/10.1007\/s13340-019-00416-7 Received: June 27, 2019; accepted December 31, 2019; published online November 19, 2019 Abstract […]","_links":{"self":[{"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/posts\/7643","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/comments?post=7643"}],"version-history":[{"count":17,"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/posts\/7643\/revisions"}],"predecessor-version":[{"id":8611,"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/posts\/7643\/revisions\/8611"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/media\/7760"}],"wp:attachment":[{"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/media?parent=7643"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/categories?post=7643"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.arkray-adams-portal.com\/en_id\/wp-json\/wp\/v2\/tags?post=7643"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}